- Overview
- Features
- Applications
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Applikon MiniBio
Real Small... Real Bioreactors
- Easy set up and operation
- Little bench space required
- Less medium used during cultivation
- Generate more data in less time
The MiniBio is the perfect device for small-scale operations in the lab. It saves time, takes up less bench space, and generates more data while providing fully scalable results. In combination with our highly efficient BioSep cell separation device, you can convert your batch or fed-batch bioreactor into a small scale continuous bioprocessing system.
The configurable head plate of the bioreactor has Luer fittings that free up space for multiple additions, sensors and fittings and ready-to-go tubing assemblies for a quick start.
Microbial and cell cultures at small scale
Sometimes less leads to more.
The Applikon MiniBio requires less medium and bench space than regular laboratory-scale bioreactors. Scientists can easily run multiple experiments in parallel, generating more data at once to enable efficient scale-up.
Watch the video to view how the MiniBio can speed up your bioprocess.
Small size bioreactors
The compact design of the MiniBio allows the scientist to place multiple systems on the valuable bench space. Parallel processing has never been that easy.
Generate scalable results
Running cultivations at small scale allows for quick access to more data. Scientists will have reliable data that simplifies scale-up to higher volumes.
Specifications
MiniBio 250 |
MiniBio 500 |
MiniBio 1000 |
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| Total volume (mL) | 290 | 550 | 1250 |
| Working volume (mL) | 200 | 400 | 1000 |
| Minimum working volume (mL) | 50 | 100 | 200 |
| Aspect ratio total volume | 2.3 | 2.1 | 2.3 |
| Aspect ratio working volume | 1.6 | 1.5 | 1.9 |
| Dimensions (dxh) | 180 x 400 mm | 195 x 400 mm | 200 x 550 mm |
| Dimensions for autoclaving (dxh) | 180 x 250 mm | 195 x 250 mm | 200 x 400 mm |
| Drive system | Direct drive, lipsealed | Direct drive, lipsealed | Direct drive, lipsealed |
| Maximum stirrer speed (rpm) | 50 - 2000 | 50 - 2000 | 50 - 1750 |
| Maximum impeller tip speed (m/s) | 2.3 | 2.9 | 3.5 |
| Impellers | Choice of Rushton and Marine | ||
| Gas sparger | Porous sparger, open pipe sparger or jet sparger | ||
| Gas overlay | Yes | ||
| Exhaust gas | Electrically cooled exhaust gas condenser (evaporation <4% per day at 37°C @ 2vvm) | ||
| Sampling | Fixed sample pipe with optional sampling system | ||
| Draining | Height adjustable drain pipe | ||
| Additions | 4 fixed inlet ports and optional micro liquid injectors | ||
| pH | Measurement: 8 mm classic pH sensor Control: via acid pump (variable speed pump) or CO2 gas in combination with alkali pump (variable speed pump) |
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| DO2 | Measurement: LumiSens Optical DO2 sensor. Control: via a combination of N2, Air, O2 (needle valve standard, MFC optional) and agitation |
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| Temperature | Measurement: Pt-100 sensor in thermowell in topplate Control: electrical cooling and heating jacket via bioreactor wall |
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| Foam | Measurement: Height adjustable conductivity based foam sensor Control: anti-foam addition (variable speed pump) |
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| Level | Measurement: Height adjustable conductivity based level sensor Control: variable speed pump for liquid addition or removal |
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| Optional inlets | Septum, chemostat tube, liquid entry system | ||
| Optional sensors | Biomass, Optical Density, O2 and CO2 off gas, weight balance | ||
The Applikon MiniBio is ideal for the following applications:
- Screening studies
- Media optimization
- Process optimization
- Microbial and cell culture
- Batch, Fed-Batch, Perfusion and Continuous cultivation