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Applikon Micro-Flask

Name: Applikon Micro-Flask
Brand: Getinge

Reproducible cultivation in microtiter plates

An easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines. This system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings.

Your interest in bioreactor systems

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The Applikon Micro-Flask with various configurations of cover clamps and sandwich covers

The Applikon Micro-Flask by Duetz design

Speed up your process by cultivating strains in parallel

Low and uniform evaporation rates
Sterile barrier for individual wells prevents cross contamination
Oxygen transfer rates similar to shake flasks in standard orbital shakers
Conversion of 24 and 96 microtiter plates (both deep and low-well plates) into individual micro-bioreactors
Simultaneous and reproducible sampling of 96 frozen glycerol stocks using our Cryo-Replicator

All handling with the Micro-Flask is performed in parallel with sets of 24 or 96 strains. It provides a more efficient use of your current orbital shaker, with time savings up to a factor 10 possible. It allows for the same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility.

Consumables & Accessories

The Applikon Micro-Flask allows for:

various square deep-well and round low-well microtiter plates
a Sandwich-cover (silicone seal, ePTFE + microfibre layer and stainless steel cover)
a clamp
a Cryo replicator and press

Individual micro-reactors

The Micro-Flask turns 6, 24 and 96-well microtiter plates into individual micro-reactors enabling simultaneous and reproducible cultivations.

Transfer of oxygen

The oxygen transfer in the Micro-Flask is similar to fermenters enabling accurate cultivations.

Specifications

Type of microtiter plate	Well volume	Culture volume	Orbital shaking frequency	Shaking amplitude	O2-transfer rate (30˚C, air, 1 bar)	Headspace refreshment rate	Evaporation rate per well (at 30˚C)	Mixing pattern at 300 rpm
								ampl. 25 mm	ampl. 50 mm
24-square deep-well polypropylene, 17x17 mm, depth 40 mm	11000 µL	2500 µL	300 rpm	50 mm	51 mmol O₂/l/h	2.5 mL / min (1 VVM)	50% humidity: 50 µL H₂O per day 75% humidity: 25 µL H₂O per day	2500 µL	2500 µL
		2500 µL	300 rpm	25 mm	39 mmol O₂/l/h
		2500 µL	220 rpm	50 mm	35 mmol O₂/l/h

		4000 µL	300 rpm	50 mm	24 mmol O₂/l/h	2.5 mL / min (0.6 VVM)
		4000 µL	220 rpm	25 mm	24 mmol O₂/l/h	2.5 mL / min (0.6 VVM)
24-round low-well polystyrene, ⌀16 mm, depth 18 mm	3000 µL	750 µL	300 rpm	50 mm	40 mmol O2/l/h	1.1 mL / min (1.4 VVM)	50% humidity: 30 µL H₂O per day 75% humidity: 15 µL H₂O per day	1000 µL	1000 µL
		750 µL	300 rpm	25 mm	25 mmol O2/l/h	1.1 mL / min (1.4 VVM)

		1000 µL	300 rpm	50 mm	30 mmol O2/l/h	1.1 mL / min (1.1 VVM)
		1000 µL	300 rpm	25 mm	19 mmol O2/l/h	1.1 mL / min (1.1 VVM)
96-square deep-well polypropylene, 8x8 mm, depth 40 mm	2400 µL	500 µL	300 rpm	50 mm	38 mmol O2/l/h	1 mL / min (2 VVM)	50% humidity: 22 µL H₂O per day 75% humidity: 11 µL H₂O per day	750 µL	750 µL
		500 µL	300 rpm	25 mm	12 mmol O2/l/h	1 mL / min (2 VVM)

		750 µL	300 rpm	50 mm	24 mmol O2/l/h	1 mL / min (1.3 VVM)
		750 µL	300 rpm	25 mm	7 mmol O2/l/h	1 mL / min (1.3 VVM)

		1000 µL	300 rpm	50 mm	18 mmol O2/l/h	1 mL / min (1 VVM)
		1000 µL	300 rpm	25 mm	3 mmol O2/l/h	1 mL / min (1 VVM)
96-round low-well polystyrene, ⌀6.5 mm, depth 11 mm	380 µL	100 µL	300 rpm	50 mm	39 mmol O2/l/h	250 µL / min (2.5 VVM)	50% humidity: 6 µL H₂O per day 75% humidity: 3 µL H₂O per day	150 µL	150 µL
		100 µL	300 rpm	25 mm	20 mmol O2/l/h	250 µL / min (2.5 VVM)

		150 µL	300 rpm	50 mm	32 mmol O2/l/h	250 µL / min (1.7 VVM)
		150 µL	300 rpm	25 mm	16 mmol O2/l/h	250 µL / min (1.7 VVM)

		200 µL	220 rpm	50 mm	12 mmol O2/l/h	250 µL / min (1.3 VVM)
		200 µL	300 rpm	25 mm	12 mmol O2/l/h	250 µL / min (1.3 VVM)

The Applikon Micro-Flask is ideal for the following applications:

Growth medium optimization studies
Comparative studies e.g. clinical isolates
Screening and distribution of clone libraries in E. coli or yeasts
High-throughput screening for high-activity or high-productivity mutants
High-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)