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Applikon Micro-Flask

Cultivo reprodutível em placas de microtitulação

Uma plataforma tecnológica econômica e fácil de usar para o rápido manuseio e crescimento de grandes números de cepas microbianas, bibliotecas clonais, bancos mutantes e linhagens celulares. Esse sistema permite que uma única pessoa cultive e teste milhares de cepas simultaneamente com um mínimo de manuseios repetitivos.

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Your interest in bioreactor systems

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Argentina

Hixwer 
Phone: +54 11 7078-6666
Email: info@hixwer.com
URL: www.hixwer.com

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Argentina

Hixwer 
Phone: +54 11 7078-6666
Email: info@hixwer.com
URL: www.hixwer.com

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Brazil

Sotelab
+55 (21) 3392-3563
www.sotelab.com.br

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Brazil

Sotelab
+55 (21) 3392-3563
www.sotelab.com.br

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Chile, Peru

AP Bioprocess
www.ap-bioprocess.com

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Chile, Peru

AP Bioprocess
www.ap-bioprocess.com

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Colombia

Intek Group
Phone: +57 316 3550400 - 749 8437
Email: contacto@intekgroup.com.co
URL: www.intekgroup.com.co

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Colombia

Intek Group
Phone: +57 316 3550400 - 749 8437
Email: contacto@intekgroup.com.co
URL: www.intekgroup.com.co

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Czech Republic

Vekamaf
Phone: +420 284 690 881
URL: www.vekamaf.cz

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Czech Republic

Vekamaf
Phone: +420 284 690 881
URL: www.vekamaf.cz

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Denmark

Holm & Halby
Phone: +454326 9400
Email: info@holm-halby.dk
URL: www.holm-halby.dk

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Denmark

Holm & Halby
Phone: +454326 9400
Email: info@holm-halby.dk
URL: www.holm-halby.dk

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Estonia

Bio Techa
Phone: +372 6597101
Email: info@biotecha.ee
URL: www.biotecha.lt

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Estonia

Bio Techa
Phone: +372 6597101
Email: info@biotecha.ee
URL: www.biotecha.lt

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Hungary

Holimex
Phone: +36 1 391 4400
URL: www.holimex.hu

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Hungary

Holimex
Phone: +36 1 391 4400
URL: www.holimex.hu

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India

Spinco Biotech Pvt. Ltd.
Phone: +91 44 45678900
Email: info@spincotech.com
URL: www.spincotech.com

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India

Spinco Biotech Pvt. Ltd.
Phone: +91 44 45678900
Email: info@spincotech.com
URL: www.spincotech.com

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Indonesia

PT Arico Sainsindo Nusantara
Phone: +62-21 580 6342 / 3
Email: arico@dnet.net.id
URL: www.ptarico.com

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Indonesia

PT Arico Sainsindo Nusantara
Phone: +62-21 580 6342 / 3
Email: arico@dnet.net.id
URL: www.ptarico.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Ireland

Asistec
Phone: +353(0)1286 8796
Email: info@asistec.ie
URL: www.asistec.ie

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Ireland

Asistec
Phone: +353(0)1286 8796
Email: info@asistec.ie
URL: www.asistec.ie

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Israel

Medent Group
Phone: +97286237984
Email: info@medent.co.il
URL: medent.co.il

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Israel

Medent Group
Phone: +97286237984
Email: info@medent.co.il
URL: medent.co.il

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Italy

Steroglass
Phone: +39 075 609091
Email: info@steroglass.it 
URL: www.steroglass.it

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Italy

Steroglass
Phone: +39 075 609091
Email: info@steroglass.it 
URL: www.steroglass.it

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Japan

Sanyo Trading Co., LTD
Phone: +81-3-3518-1196
URL: www.sanyo-trading.co.jp

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Japan

Sanyo Trading Co., LTD
Phone: +81-3-3518-1196
URL: www.sanyo-trading.co.jp

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Lithuania

Bio Techa
Phone: +370 5 237 60 07
Email: info@biotecha.lt
URL: www.biotecha.lt

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Lithuania

Bio Techa
Phone: +370 5 237 60 07
Email: info@biotecha.lt
URL: www.biotecha.lt

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Norway

Bio Nordica
Phone: 23 03 58 00
Email: info@bionordika.no
URL: bionordika.no

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Norway

Bio Nordica
Phone: 23 03 58 00
Email: info@bionordika.no
URL: bionordika.no

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Poland

Labo Baza
Phone: +4861 812 57 45
Email: biuro@labobaza.pl
URL: www.labobaza.pl

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Poland

Labo Baza
Phone: +4861 812 57 45
Email: biuro@labobaza.pl
URL: www.labobaza.pl

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Portugal

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

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Portugal

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

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Russia

Abtek
Phone: +7(495) 723-64-97
Email: abtek@abtek.ru
URL: abtek.ru

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Russia

Abtek
Phone: +7(495) 723-64-97
Email: abtek@abtek.ru
URL: abtek.ru

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Singapore

Zil Sci Pte. Ltd.
Phone: +65 6767 2929
Email: ENQUIRY@ZILSCI.COM
URL: www.zilsci.com

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Singapore

Zil Sci Pte. Ltd.
Phone: +65 6767 2929
Email: ENQUIRY@ZILSCI.COM
URL: www.zilsci.com

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Slovakia

Vekamaf
Phone: +421-2-52932891
URL: www.vekamaf.cz

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Slovakia

Vekamaf
Phone: +421-2-52932891
URL: www.vekamaf.cz

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South Africa

Seperations
Phone: +27 11 919 1000
Email: info@separations.co.za
URL: separations.co.za

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South Africa

Seperations
Phone: +27 11 919 1000
Email: info@separations.co.za
URL: separations.co.za

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Korea

SeouLin Bioscience
Phone: +821670-5911
Email: support@seoulin.co.kr
URL: www.seoulin.co.kr

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Korea

SeouLin Bioscience
Phone: +821670-5911
Email: support@seoulin.co.kr
URL: www.seoulin.co.kr

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Spain

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Spain

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Switzerland

Resea Biotec
Phone: +41 34 531 08 20
Email: office@reseabiotec.ch
URL: www.reseabiotec.ch

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Switzerland

Resea Biotec
Phone: +41 34 531 08 20
Email: office@reseabiotec.ch
URL: www.reseabiotec.ch

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Taiwan

Tseng Hsiang Life Science Ltd.
Phone: +862-27851156
Email: info@thco.com.tw
URL: www.thco.com.tw

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Taiwan

Tseng Hsiang Life Science Ltd.
Phone: +862-27851156
Email: info@thco.com.tw
URL: www.thco.com.tw

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Meditop Co., Ltd

Holm & Halby
Phone: +662 9331133
Email: info@meditopthailand.com
URL: meditopthailand.com

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Meditop Co., Ltd

Holm & Halby
Phone: +662 9331133
Email: info@meditopthailand.com
URL: meditopthailand.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Turkey

Ant Teknik
Phone: +90 216 422 6700
Email: antteknik@antteknik.com 
URL: www.antteknik.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Turkey

Ant Teknik
Phone: +90 216 422 6700
Email: antteknik@antteknik.com 
URL: www.antteknik.com

The Applikon Micro-Flask with various configurations of cover clamps and sandwich covers
The Applikon Micro-Flask by Duetz design

Acelere seu processo cultivando cepas em paralelo

  • Taxas de evaporação baixas e uniformes
  • A barreira estéril para poços individuais evita a contaminação cruzada
  • Taxas de transferência de oxigênio semelhantes às de frascos de agitação em agitadores orbitais padrão
  • Conversão de placas microtituladoras de 24 e 96 (tanto profundas quanto baixas) em microbiorreatores individuais
  • Amostragem simultânea e reprodutível de 96 estoques de glicerol congelados usando nosso criorreplicador

Todo o manuseio com o Micro-Flask é realizado em paralelo a conjuntos de 24 ou 96 cepas. Ele proporciona um uso mais eficiente do seu agitador orbital atual, com possibilidade de economia de tempo de até 10 vezes. Em termos de taxas de transferência de oxigênio e reprodutibilidade, ele permite a mesma qualidade de crescimento dos frascos Erlenmeyer.

Produtos relacionados

Microrreatores individuais

O Micro-Flask transforma as placas de microtitulação de 6, 24 e 96 poços em microrreatores individuais, permitindo um cultivo simultâneo e reprodutível.

Transferência de oxigênio

A transferência de oxigênio no Micro-Flask é semelhante aos fermentadores, permitindo um cultivo preciso.

Especificações

Tipo de
placa de microtitulação
Volume do poço Volume da cultura Frequência de agitação orbital Amplitude de agitação Taxa de transferência de O2
(30 °C, ar, 1 bar)
Taxa de renovação do espaço livre Taxa de evaporação por poço
(a 30 °C)
Padrão de mistura
a 300 rpm
             

 

ampl. de
25 mm
ampl. de
50 mm
24 poços quadrados fundos
em polipropileno,
17x17 mm,
profundidade de 40 mm
11.000 μL 2.500 μL 300 rpm 50 mm 51 mmol O2/L/h 2,5 mL/min
(1 VVM)

50% de umidade:
50 μL de H2O por dia

75% de umidade:
25 μL de H2O por dia

2.500 μL 2.500 μL
2.500 μL 300 rpm 25 mm 39 mmol O2/L/h
2.500 μL 220 rpm 50 mm 35 mmol O2/L/h
         
4.000 μL 300 rpm 50 mm 24 mmol O2/L/h 2,5 mL/min
(0,6 VVM)
4.000 μL 220 rpm 25 mm 24 mmol O2/L/h
24 poços redondos rasos
de poliestireno, ⌀16 mm,
profundidade de 18 mm
3.000 μL 750 μL 300 rpm 50 mm 40 mmol O2/L/h 1,1 mL/min
(1,4 VVM)

50% de umidade:
30 μL de H2O por dia

75% de umidade:
15 μL de H2O por dia

1.000 μL 1.000 μL
750 μL 300 rpm 25 mm 25 mmol O2/L/h
         
1.000 μL 300 rpm 50 mm 30 mmol O2/L/h 1,1 mL/min
(1,1 VVM)
1.000 μL 300 rpm 25 mm 19 mmol O2/L/h
96 poços quadrados fundos
em polipropileno,
8x8 mm,
profundidade de 40 mm
2.400 μL 500 μL 300 rpm 50 mm 38 mmol O2/L/h 1 mL/min
(2 VVM)

50% de umidade:
22 μL de H2O por dia

75% de umidade:
11 μL de H2O por dia

750 μL 750 μL
500 μL 300 rpm 25 mm 12 mmol O2/L/h
         
750 μL 300 rpm 50 mm 24 mmol O2/L/h 1 mL/min
(1,3 VVM)
750 μL 300 rpm 25 mm 7 mmol O2/L/h
         
1.000 μL 300 rpm 50 mm 18 mmol O2/L/h 1 mL/min
(1 VVM)
1.000 μL 300 rpm 25 mm 3 mmol O2/L/h
96 poços redondos rasos
de poliestireno,
⌀6,5 mm,
profundidade de 11 mm
380 μL 100 μL 300 rpm 50 mm 39 mmol O2/L/h 250 μL/min
(2,5 VVM)

50% de umidade:
6 μL de H2O por dia

75% de umidade:
3 μL de H2O por dia

150 μL 150 μL
100 μL 300 rpm 25 mm 20 mmol O2/L/h
         
150 μL 300 rpm 50 mm 32 mmol O2/L/h 250 μL/min
(1,7 VVM)
150 μL 300 rpm 25 mm 16 mmol O2/L/h
         
200 μL 220 rpm 50 mm 12 mmol O2/L/h 250 μL/min
(1,3 VVM)
200 μL 300 rpm 25 mm 12 mmol O2/L/h